The tweet that kicked off this project.
Next steps: mitotracker + fluorescence + confocal. Try with buccal cells and WBC. If success, try with 10 subjects.
Latest Update: [x] janus green experiments. [x] fluorescence microscopy.
Newest Video: Added minimap to Mitosite
Newest Image:
Cheek cells. dye: 1% methylene blue.
The cast that started it.
I'm going to setup my own home lab for imaging mitochondria. I'm going to do it as cheaply and reproducibly as possible so anyone can copy the setup. The goal is to create the world's largest public domain database of mitochondria images. Follow me if interested!
1/28/2025 - MitoView™ Mitochondrial Dyes - Trial size (50 ug), MitoView™ Green ordered
1/27/2025 - first confocal fluorescence
1/24/2025 - visit cc microscopy lab.
1/24/2025 - janus green arrived.
1/23/2025 - visit dlab. first oil immersion session
1/21/2025 - added zoom views to Mitosite
1/20/2025 - methlyene blue 1% stain images
1/20/2025 - mitosite rev 2
1/16/2025 - named finalist at Nvidia hackathon!
1/16/2025 - started Mitosite at Nvidia hackathon.
1/15/2025 - MBC safety training complete
1/15/2025 - Published https://receipts.powerhouse.wiki
1/14/2025 - Purchased Powerhouse.wiki ($3.16)
1/09/2025 - First videos
1/09/2025 - MBC demo
12/27/2024 - First sp images
12/24/2024 - RBC and White blood cell images. (todo: figure out WBC separation)
12/23/2024 - Microscope arrived. First images.
12/19/2024 - Purchased AmScope 72 Pre-Cleaned Blank Microscope Slides and 100 22x22mm Square Cover Glass. ($11.99)
12/19/2024 - Purchased Swift 1.3 Megapixel Digital Camera for Microscopes, Eyepiece Mount, USB 2.0 Connection, Color Photography and Video, Windows and Mac Compatible ($48.99)
12/19/2024 - Purchased SWIFT Compound Monocular Microscope SW200DL ordered. ($89.99)
12/17/2024 - MBC tour
12/12/2024 - Funding secured! Thanks to the Warpcast community, we launched $MITO and raised over $2,000 to fund the PowerHouse Project.
12/12/2024 - Added mitocounter script.
12/12/2024 - Website launched
12/12/2024 - @Ayyy_Unit tweets name idea
12/12/2024 - Breck tweets idea
Cheek cell. Janus green stain.
Cheek cell. Janus green stain.
For ballpark scale, overlaid fluorescent light micrograph of cultured human cells (source).
Calibrating with a stage micrometer.
Cheek cells. dye: 1% methylene blue.
Red blood cells, 1000x.
Cheek cell, 1000x.
This script (mitocounter.py) can be used to take an image of stained mitochondria and count them.
Example input:
Example output:
Are there cheap fluorescence microscopes?
Can we build our own fluorescence microscopes? We only need 1 range.
Likely going to need a scope with oil immersion
Beginners watch this (@micro_safari - microsafari.org)
Suppliers:
Rhodamine 123
Thermofischer Scientific - 25mg for $139.65
Janus Green B
methylene blue
Cheek (buccal) epithelial cells
Light microscopy of mitochondria at the nanoscale
Analysis of mitochondrial dynamics and functions using imaging approaches
Live cell microscopy of mitochondria-lysosome contact site formation and tethering dynamics
Mitochondrial Dynamics in the Regulation
of Nutrient Utilization and Energy Expenditure
Mitochondrial volume densities in the smokeless
tobacco-treated hamster cheek pouch epithelium
~1 micron in diameter
Symposium on Mitochondria at the School of Medicine, Westlake University
6th Annual Mitochondria Symposium
https://x.com/Mito_Therapy/status/1868212807965000114
https://x.com/RDennerlein_Lab/status/1841429716810477715
Can we rig up a spectrophotometer approach? Do we need imaging, or can we get by with just dye + color reading?
$89.99Swift Microscope | $48.99Swift 1.3 Megapixel Digital Camera | TBDCustom Dye |
Just a place to write down names of folks providing help/advice.
Clone this site:
git clone https://powerhouse.breckyunits.com/powerhouse.breckyunits.com.git
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