Build your own at-home Mitochondria Imaging Lab
I'm going to setup my own home lab for imaging mitochondria. I'm going to do it as cheaply and reproducibly as possible so anyone can copy the setup. The goal is to create the world's largest public domain database of mitochondria images. Follow me if interested!
Mitocounter
This script (mitocounter.py) can be used to take an image of stained mitochondria and count them.
Example input:
Example output:
Materials
Microscope
Preparation & Dyes
Specimen
Microscope Research
Are there cheap fluorescence microscopes?
Can we build our own fluorescence microscopes? We only need 1 range.
Likely going to need a scope with oil immersion
Beginners watch this (@micro_safari - microsafari.org)
Suppliers:
Potential Dyes
Rhodamine 123
Thermofischer Scientific - 25mg for $139.65
Todo
Papers
Optical
Light microscopy of mitochondria at the nanoscale
Confocal
Analysis of mitochondrial dynamics and functions using imaging approaches
Live cell microscopy of mitochondria-lysosome contact site formation and tethering dynamics
Mitochondria
~1 micron in diameter
Links
Biohacking resources
Electron microscope
Labs
Scientific Questions
- Are changes in quantity of mitochondria is reflected in changes in membrane potential?
- IMO you should also at least do something with JC-1 or TMRE to see if any changes in quantity of mitochondria is reflected in changes in membrane potential. - CRISPR_cat9 on Reddit
Project History
12/12/2024 - Funding secured! Thanks to the Warpcast community, we launched $MITO and raised over $2,000 to fund the PowerHouse Project.
12/12/2024 - Added mitocounter script.
12/12/2024 - Website launched
12/12/2024 - @Ayyy_Unit tweets name idea
12/12/2024 - Breck tweets idea
Clone this site:
git clone https://powerhouse.breckyunits.com/powerhouse.breckyunits.com.git