Project PowerHouse

The tweet that kicked off this project.

Next steps: mitotracker + fluorescence + confocal. Try with buccal cells and WBC. If success, try with 10 subjects.

Latest Update: [x] janus green experiments. [x] fluorescence microscopy.

Newest Video: Added minimap to Mitosite

Newest Image:

Cheek cells. dye: 1% methylene blue.

  1. Project origin
  2. Build your own at-home Mitochondria Imaging Lab
  3. Project History
  4. Images
  5. Mitocounter
  6. Materials
  7. -Microscope
  8. -Preparation & Dyes
  9. -Specimen
  10. Microscope Research
  11. Potential Dyes
  12. Cell Types
  13. Todo
  14. Papers
  15. -Optical
  16. -Confocal
  17. Mitochondria
  18. Electron microscope
  19. Labs
  20. Spaces
  21. Conferences
  22. Links
  23. Twitter Accounts to Follow
  24. Scientific Questions
  25. Broadband near-infrared spectroscopy
  26. Spectrophotometry
  27. V0
  28. Thanks
  29. Phase 2
  30. Jobs

Project origin

The cast that started it.

Build your own at-home Mitochondria Imaging Lab

I'm going to setup my own home lab for imaging mitochondria. I'm going to do it as cheaply and reproducibly as possible so anyone can copy the setup. The goal is to create the world's largest public domain database of mitochondria images. Follow me if interested!

Project History

1/28/2025 - MitoView™ Mitochondrial Dyes - Trial size (50 ug), MitoView™ Green ordered

1/27/2025 - first confocal fluorescence

1/24/2025 - visit cc microscopy lab.

1/24/2025 - janus green arrived.

1/23/2025 - visit dlab. first oil immersion session

1/21/2025 - added zoom views to Mitosite

1/20/2025 - methlyene blue 1% stain images

1/20/2025 - mitosite rev 2

1/16/2025 - named finalist at Nvidia hackathon!

1/16/2025 - started Mitosite at Nvidia hackathon.

1/15/2025 - MBC safety training complete

1/15/2025 - Published https://receipts.powerhouse.wiki

1/14/2025 - Purchased Powerhouse.wiki ($3.16)

1/09/2025 - First videos

1/09/2025 - MBC demo

12/27/2024 - First sp images

12/24/2024 - RBC and White blood cell images. (todo: figure out WBC separation)

12/23/2024 - Microscope arrived. First images.

12/19/2024 - Purchased AmScope 72 Pre-Cleaned Blank Microscope Slides and 100 22x22mm Square Cover Glass. ($11.99)

12/19/2024 - Purchased Swift 1.3 Megapixel Digital Camera for Microscopes, Eyepiece Mount, USB 2.0 Connection, Color Photography and Video, Windows and Mac Compatible ($48.99)

12/19/2024 - Purchased SWIFT Compound Monocular Microscope SW200DL ordered. ($89.99)

12/17/2024 - MBC tour

12/12/2024 - Funding secured! Thanks to the Warpcast community, we launched $MITO and raised over $2,000 to fund the PowerHouse Project.

12/12/2024 - Added mitocounter script.

12/12/2024 - Website launched

12/12/2024 - @Ayyy_Unit tweets name idea

12/12/2024 - Breck tweets idea

Images

Cheek cell. Janus green stain.

Cheek cell. Janus green stain.

For ballpark scale, overlaid fluorescent light micrograph of cultured human cells (source).

Calibrating with a stage micrometer.

Cheek cells. dye: 1% methylene blue.

Red blood cells, 1000x.

Cheek cell, 1000x.

Mitocounter

This script (mitocounter.py) can be used to take an image of stained mitochondria and count them.

Example input:

Example output:

Materials

Microscope

Preparation & Dyes

Specimen

Microscope Research

Are there cheap fluorescence microscopes?

Can we build our own fluorescence microscopes? We only need 1 range.

Likely going to need a scope with oil immersion

Beginners watch this (@micro_safari - microsafari.org)

Suppliers:

AmScope

Potential Dyes

Rhodamine 123

Thermofischer Scientific - 25mg for $139.65

bitium - 50mg for $116

Janus Green B

methylene blue

Cell Types

Cheek (buccal) epithelial cells

Todo

Electron microscope

Broadband near-infrared spectroscopy

Spectrophotometry

Can we rig up a spectrophotometer approach? Do we need imaging, or can we get by with just dye + color reading?

Nix Mini 3 for $89

V0

$89.99Swift Microscope $48.99Swift 1.3 Megapixel Digital Camera TBDCustom Dye

Thanks

Just a place to write down names of folks providing help/advice.

  • $MITO supporters
  • Chrisin
  • Paula
  • Cedric
  • Lambert

Phase 2

Clone this site:

git clone https://powerhouse.breckyunits.com/powerhouse.breckyunits.com.git

Jobs

Sales - BY & ?

Software, Data & Research - BY

Recruiting - BY & ?

Hardware - ?

AI - ?

Grants & Fundraising - SR & BY & ?

Organization - ?

Marketing & Branding - ?